Evaluation of the Vysis IntelliFISH Hybridization Buffer and Vysis IntelliFISH Universal FFPE Pretreatment and Wash Kit in FISH Assays

Standard fluorescence in-situ hybridization (FISH) technique requires a hybridization time of 12 hours or greater, thus amounting to a turn-around time (TAT) of 24 hours or greater for result reporting. To improve the TAT for the FISH tests, a new Vysis IntelliFISH Hybridization Buffer has been recently developed by Abbott Molecular. It was evaluated in 20 pairs of matched bone marrow samples and 10 pairs of matched formalin-fixed lymphoma tissue samples against the standard Vysis LSI/WCP Hybridization Buffer protocol. Compared to the standard Vysis LSI/WCP Hybridization Buffer protocol, the new fast working Vysis IntelliFISH Hybridization Buffer protocol reduced FISH hybridization time and simplified the workflow of the standard overnight hybridization protocol. In addition, signal intensity, slide background and signal specificity of FISH probes were comparable to those generated with the standard hybridization protocol. [N A J Med Sci. 2017;10(1):5-7. DOI: 10.7156/najms.2017.1001005]